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Spiny versus stubby: 3D reconstruction of human myenteric (type I) neurons
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1Tobias M. Lindig, 2Vinod Kumar, 1Ron Kikinis, 4Steve Pieper, 1Falk Schrödl, 1Winfried L. Neuhuber, 1Axel Brehmer
Institution: |
1Department of Anatomy I, University of Erlangen-Nuremberg, Krankenhausstrasse 9, 91054, Erlangen, Germany. 2Section of Experimental Magnetic Resonance of the CNS, University of Tuebingen, 72076 Tuebingen, Germany 3Surgical Planning Laboratory, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA. 4Isomics Inc., Cambridge, MA 02115, USA |
Publisher: |
Histochem Cell Biol |
Publication Date: |
Jan-2009 |
Citation: |
Histochem Cell Biol. 2009 Jan;131(1):1-12. |
PubMed ID: |
18807064 |
PMCID: |
PMC2756529 |
Keywords: |
Neuron type, Confocal microscopy, Gut, Enteric nervous system, Innervation |
Appears in Collections: |
SLICER, NA-MIC, NAC, SPL |
Sponsors: |
P41 RR13218/RR/NCRR NIH U54 EB005149/EB/NIBIB NIH |
Generated Citation: |
Lindig T, Kumar V, Kikinis R, Pieper S, Schrödl F, Neuhuber W, Brehmer A. Spiny versus stubby: 3D reconstruction of human myenteric (type I) neurons. Histochem Cell Biol. 2009 Jan;131(1):1-12. PMID: 18807064. PMCID: PMC2756529. |
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We have compared the three-dimensional (3D) morphology of stubby and spiny neurons derived from the human small intestine. After immunohistochemical triple staining for leu-enkephalin (ENK), vasoactive intestinal peptide (VIP) and neurofilament (NF), neurons were selected and scanned based on their immunoreactivity, whether ENK (stubby) or VIP (spiny). For the 3D reconstruction, we focused on confocal data pre-processing with intensity drop correction, non-blind deconvolution, an additional compression procedure in z-direction, and optimizing segmentation reliability. 3D Slicer software enabled a semi-automated segmentation based on an objective threshold (interrater and intrarater reliability, both 0.99). We found that most dendrites of stubby neurons emerged only from the somal circumference, whereas in spiny neurons, they also emerged from the luminal somal surface. In most neurons, the nucleus was positioned abluminally in its soma. The volumes of spiny neurons were significantly larger than those of stubby neurons (total mean of stubbies 806±128 μm3, of spinies 2,316±545 μm3), and spiny neurons had more dendrites (26.3 vs. 11.3). The ratios of somal versus dendritic volumes were 1:1.2 in spiny and 1:0.3 in stubby neurons. In conclusion, 3D reconstruction revealed new differences between stubby and spiny neurons and allowed estimations of volumetric data of these neuron populations.
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